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Isolation of plasma, DNA, RNA and protein Blood samples were centrifuged at 1000 g for 10 minutes at 4C to isolate plasma

Bioinformatics analyses using the Broad Institute Connectivity Map identified GHK-Cu as a modulator of more than 4,000 human genes, with the expression signature appearing to reverse patterns associated with aging and age-related disease (PMID: 25904764)