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Serum samples (200 L) were thawed and extracted with 600-L acetonitrile:formic acid (98:2, v/v) by vortexing for 2 min and ultrasonic bath for 10 min

These mechanisms remain preclinical , and there is no MHRA-approved evidence demonstrating these effects in humans for bodybuilding, weight loss, or anti-ageing purposes

Comprehensive application of GSH chemical binding and enzymatic binding models The GSH chemical binding and enzymatic binding models are collectively termed the GSH binding model, enabling more accurate interpretation of relevant data in the research and development of covalent inhibitors