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Increased muscle PGC-1 expression protects from sarcopenia and metabolic disease during aging

K.ChakravartyB

In fact, the hypoxia factor HIF1 activates the transcription of the BNIP3 gene, 116 encoding the BNIP3 protein, which can induce autophagy

In immunoprecipitation experiments, cells were lysed in IP buffer [25 mM Hepes (pH 7.4), 150 mM NaCl, 10 mM Na 3 PO 4 , 2.7 mM KCl, 0.5% CHAPS, 1 mM DTT, and a protease inhibitor cocktail]