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Hypoxia and the hypoxia-inducible-factor pathway in glioma growth and angiogenesis

Diekstra FP, Saris CG, van Rheenen W, Franke L, Jansen RC, van Es MA, et al

The supernatant was carefully removed and the pellet rinsed three times in 75% ethanol, centrifuged at 12,000 rpm for 15 min and then allowed to air dry