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The afferent input generated by formalin is believed to release excitatory amino acids and peptides as inflammatory signaling molecules, which, through NMDA and NK1 receptors, initiate a cascade

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Patch pipettes (4-6 Mohm) were filled with cesium solution containing 115 mM cesium methanesulfonate, 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 10 mM disodium phosphocreatine, 5 mM tetraethylammonium chloride, 3 mM adenosine 5-triphosphate magnesium, 2.8 mM sodium chloride, 0.5 mM guanosine 5-triphosphate sodium, and 0.4 mM ethylene glycol tetraacetic acid (pH 7.2, 285-290 mOsm/kg)

Iyer NV, Kotch LE, Agani F, Leung SW, Laughner E, Wenger RH, et al

The cysteine S -conjugate -lyases have been reviewed in this journal previously (Cooper and Pinto in Amino Acids 30:115, 2006)

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