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(2) the ability of the TJ to selectively allow paracellular transport of certain ion species acting as ion channel and (3) the ability of the TJs to maintain cell polarity, by blocking the free diffusion of proteins and lipids between the apical and basolateral domains of the plasma membrane

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Cons : Needs to be done outdoors or in a spray chamber

Use a new, sterile needle and syringe for every draw