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The second is dose and route translation : even where an effect is real in a mouse, the human-equivalent dose is not a simple body-weight scaling, and as the route section above stressed there is no human bioavailability figure to anchor an oral-versus-injected comparison

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Tap water contains impurities and microbes that can degrade the compound and contaminate your solution

The answer is yes

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Heffernan M, Summers RJ, Thorburn A, et al