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Nat Rev Dis Primers

4 Materials and methods 4.1 Cell culture and in vivo experiments 4.1.1 Cell lines Four human NB cell lines were used in this paper: Be(2)-C (MNA), IMR5-/75-shMYCN (29) (MNA, doxycycline-inducible shRNA targeting MYCN, resulting in MYCN downregulation), IMR-32 (MNA), and SH-SY5Y/6TR(EU)/pTrex-Dest-30/MYCN (non-MNA, doxycycline-inducible transgene, resulting in MYCN overexpression)

Keywords: MOTS-c, mitochondrial-derived peptide, therapeutic exploitation, synthetic biology, endocrine Introduction Mitochondria are organelles produced by archaea that are required for the production of ATP (1)

This selectivity profile reflects the unique geometry of the allosteric pocket in the GLP-1 receptor TMD, which differs in shape, size, and residue composition from the corresponding regions of related receptors

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