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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution

It's not a common protocol but the logic is sound: each route delivers BPC-157 to different tissue compartments

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We treated the cells with TGF-2, or the ALK-5 inhibitor, SD-208, or the c-MET inhibitor, EMD1214063