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We obtained a total of 60,687,163 reads for 16S rRNA gene sequencing (mean of 124,063 reads per sample in toddlers, 122,020 reads per sample in adolescents and 117,555 reads per sample in adults) and a total of 216,779,268 reads for shotgun metagenomics (mean of 485,157 reads per sample in toddlers, 411,977 reads per sample in adolescents and 425,492 reads per sample in adults) (Supplementary Tables S2B-S3)

The homogenate was centrifuged twice at 600 g for 10 min at 4 C to remove the pellet containing nuclear and debris fractions

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Michael Gleeson, Dosing and Efficacy of Glutamine Supplementation in Human Exercise and Sport Training, The Journal of Nutrition, Volume 138, Issue 10, October 2008, Pages 2045S2049S, Perna S, Alalwan TA, Alaali Z, Alnashaba T, Gasparri C, Infantino V, Hammad L, Riva A, Petrangolini G, Allegrini P, Rondanelli M

5 (download TIFF Unprocessed western blots

1 2 3 "What Are the Signs and Symptoms of Bronchiectasis?"