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If there is an imbalance in the concentration of the carrier ion on either side of the membrane, then the core channel conductance will almost certainly rectify it will have a higher conductance in the downhill direction of the concentration gradient than in the uphill direction

Achan J, Talisuna AO, Erhart A, Yeka A, Tibenderana JK, Baliraine FN, Rosenthal PJ, D'Alessandro U

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For protein expression, a 1:100 dilution of the preculture was done in 2xTY (16 g tryptone, 10 g yeast extract, 5 g NaCl per liter)

doi: 10.4103/0019-509X.175573