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Purification of DYKDDDDK-tagged Proteins Expressed in Mammalian Cells This protocol depicts an efficient method to obtain high yields for purification of up to 10 mg of DYKDDDDK-tagged recombinant protein/ antibody from ~200 to ~900 ml tissue culture supernatant

Although rat, hamster, mouse, bovine, and chicken rhodanese have the conserved Lys 13 , human rhodanese has Gln 13 , which is similar to 3MST (55)

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Contrarily, we recently demonstrated that the new initial target is the tongue, resulting in spontaneous reversal of strong alcohol-stomach lesions

Tgfb1 (F: AAGTTGGCATGGTAGCCCTT, R: GCCCTGGATACCAACTATTGC)