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Add 1 mL of Reagent I (the proportion of tissue and reagents can be kept constant), fully grinding on ice (using liquid nitrogen will have a better grinding effect)

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GO/MF terms with high representation ratios (which is defined as the number of upregulated genes for a given GO/MF relative to the total number of genes of that GO/MF) in combination with false discovery rate (FDR) and fold enrichment metrics have been used to distinguish strong functional relevance

We first identified decreased levels of antioxidant-related DEGs such as CAT , TXNRD3 , and GPX4 and total GSH levels that determine cellular redox potential were reduced by monobromobimane (mBBr) staining in the absence of glutamine, suggesting that glutamine metabolism inhibition only triggers oxidative stress (Fig

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Stem Cell Transl Med