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BD Biosciences, #553142) for 10 min on ice and stained with conjugated antibodies in FACS buffer for 30 min (see Supplementary Table 3)

T cells migrate to the ischemic region in an antigen-independent fashion within days following acute ischemia and continue to increase until up to 28 days after stroke [27,28,37]

On-site sampling kit and laboratory analysis fees of $40 are payable to LDH/OPH/Lab with a check or money order only