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The supernatant obtained after centrifugation was incubated overnight (4 C, vertical mixer) with 2-5 g of antibodies, followed by addition of 30-40 l of Pure Proteome Protein A/G Mix Magnetic Beads (Millipore, LSKMAGAG02) at room temperature for 2-4 h

Ricketts SL, Rensing KL, Holly JM et al (2011) Prospective study of insulin-like growth factor-I, insulin-like growth factor-binding protein 3, genetic variants in the IGF1 and IGFBP3 genes and risk of coronary artery disease