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The method, in generating a non-stochastic range of single amino acid substitutions, can comprise performing amplification reactions on the nucleic acid of step (a) using the set of oligonucleotides to generate a set of variant nucleic acids encoding nineteen amino acid substitution variants at a single amino acid residue of the antibody

This transcriptional regulatory mechanism represents an important pathway for cells to enhance cystine uptake, promote glutathione synthesis, and thus respond to oxidative damage and maintain cellular redox homeostasis

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Based on our previous findings, we hypothesized that the metabolically active plasma exosomal CYP2E1 cargo participates in cellular pathophysiology and contributes to ALC- and APAP-induced toxicity in hepatic and non-hepatic cells

2011;6 doi: 10.1371/journal.pone.0026891