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Equal amounts of protein (20 g) were loaded into each lane, separated in 10% NuPAGE BT gels (Invitrogen), transferred to a PVDF membrane (Millipore), and probed with antibodies against cytochrome c (1:500, Millipore), caspase-9 (1:1000, Cell Signaling), or caspase-8 (1:1000, Cell Signaling)

No large-scale Phase III clinical trials have been completed in humans, and long-term safety data in human subjects is lacking

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