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Tissue samples and immunohistochemistry (IHC) Archived formalin-fixed, paraffin-embedded HCC samples were collected from patients diagnosed with primary hepatocellular liver cancer after radical HCC resection at Affiliated LiHuiLi Hospital of Ningbo University from January 2020 to August 2022

It is important to note thatthis trend differs from human studies, which have reported reduced GPX4 expression in renal tissue biopsies of individuals with CKD (16)