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Antibody-bound bead techniques were reported in refs 5, 18, 19, 24, 33 and 34

Trx-mGH inclusion body pellets (from equal volumes of culture medium) were resuspended in equal volumes of resuspension buffer (50 mM TrisHCl, 2 M urea, pH 10.5) and were subjected to either one or three cycles of freezethaw at 20 C or solubilised at 4 C overnight (Fig

10.1038/ncpgasthep0259 90 FerrierL.MazelinL.CenacN.DesreumauxP.JaninA.EmilieD.et al

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Oncotarget 6 (27), 2439324403