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To further elucidate its mechanism in asthma-related ferroptosis, we performed virtual docking screening combined with molecular dynamics simulations, which revealed that luteolin binds effectively to ALOX15 and maintains the protein in a relatively stable conformation, thereby potentially mitigating ferroptosis in asthma by inhibiting ALOX15

Unexpectedly, activation of p21, ICAM1, or SRXN1 was not observed (Supplementary Figure S5), although DNA (Nassini et al., 2010), and Keap1 ( Figure 6 ) is consistent with the lack of activation of an adaptive oxidative stress response pathway observed here and in a previous study (Tafazoli and OBrien, 2009)