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The membrane was blocked with 3% (w/v) milk in TBS-T [20 mM TrisHCl (pH 7.5), 150 mM NaCl, 0.1% (v/v) Tween-20] for 30 min at RT and incubated with primary antibodies (diluted in 3% milk/TBS-T) overnight at 4 C

10.2174/1381612828666220902123755 44 FreundR

[DOI] [PMC free article] [PubMed] [Google Scholar] 207d

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Having determined the molecular structure of CEP192 bound to Aurora-A, which wraps around the N-lobe of the kinase, burying a large surface area, we next compared the structure with previously determined structures of Aurora-A in mitotic complexes