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Cells were fixed by vortexing with simultaneous addition of Complete Cell Fixation Solution (Fixation Reagent1, ice-cold 100% methanol, Stanlab, Poland) with DEPC, followed by 15 min incubation on ice and then washed with WRS Buffer (Scale Biosciences) and resuspended in WRS Buffer

UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003)