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doi:10.5455/jpma.40329

The mechanism of action of GHK-Cu is versatile and remarkably broad

Upon external stimulation, signaling molecules bind to their receptors, leading to the activation of the IKK complex (via phosphorylation of IKK), which phosphorylates IB, causing its degradation and dissociation from NF-B dimers

Sperandio S, Poksay K, de Belle I, Lafuente MJ, Liu B, Nasir J, et al

Homogenised tissue was filtered through a 40 m sieve (Corning, Massachusetts, United States), centrifuged at 500 g for 5 min at 4 C to pellet the nuclei and resuspended in buffer supplemented with 1% BSA and 0.2 U/l RNAase inhibitor (Takara Bio, Kusatsu, Japan)

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