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Briefly, rice leaves were ground into powder in liquid nitrogen and resuspended in 1 ml of extraction buffer (10 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40), supplemented with 1 mM PMSF, 10 l of protease inhibitor cocktail (Sigma, P8340), and 10 l of phosphatase inhibitor cocktail 3 (Sigma, 524627)

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Brain Res 1185:256265 Nagano I, Ilieva H, Shiote M, Murakami T, Yokoyama M, Shoji M, Abe K (2005) Therapeutic benefit of intrathecal injection of insulin-like growth factor-1 in a mouse model of amyotrophic lateral sclerosis