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However, as no consensus palmitoylation sequence motifs have yet been identified, the mechanism of zDHHC-PAT substrate specificity is only poorly understood at present (52)

Afterward, 50 l of luciferin generation reagent was added to all wells and incubated at room temperature for 30 min

The morning and evening doses can be adjusted separately if needed

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Proteins containing thiol-disulfide bonds play crucial roles in regulating cellular redox homeostasis and serve as diagnostic markers for diseases influenced by redox conditions (Hanchapola et al., 2023)