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Fermented milks containing Limosilactobacillus J20 and J23 that were shown as the most proteolytic strains, presented angiotensin converting enzyme, thrombin enzyme and micellar solubility of cholesterol inhibitory activities and potential cardio-protective effects (46)

The sample tube was centrifuged at 18,000 g at 4 C for 20 min, and the supernatant containing the protein sample was collected

doi: 10.1128/microbiolspec.TNMI7-0024-2016 7 WallaceRJIakhiaevaEWilliamsMDBrown-ElliottBAVasireddySVasireddyRet al

The typical protocol of 2-5 mg twice weekly translates to 16-40 mg monthly usage

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Specificity was verified by melting curve analyses and agarose gel electrophoresis of the amplicon