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This is considered to be a steady-state situation, at which a linear back-extrapolation procedure can be used to estimate the size of the initial RRP ( y-axis intercept) corrected for vesicle recruitment

Cautions: Consult a healthcare practitioner prior to use if you are pregnant or breastfeeding

In HeLa cells, SPCA1 is required for transport of Mn into the Golgi, followed by secretion via exocytosis as a bona-fide Mn efflux pathway [69]

All reagents used above are of analytically pure

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