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The main consideration with the pen and the thigh is ensuring you are placing it on the correct zone (front or outer, middle third) and not pressing too hard

Once reconstituted for in-vitro work, follow your laboratory's standard handling and stability protocols

For example, certain biological products or drugs (like some vaccines or delicate protein-based drugs) should not be reconstituted with bacteriostatic water because the preservative could interfere with the drugs stability or efficacy

a review Eur J Endocrinol 1998 139 : 19

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This commenter further urged CMS to allow RHC's to be able to conduct an AWV the same day as an office visit as they noted they believe it is often difficult either logistically or financially for many in the rural healthcare setting to make 2 trips, and as a result they do not believe as many patients whose only access to healthcare that is being served by a RHC are benefiting from AWV's