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Like other copper-binding tripeptides, its biological effects are proposed to occur through both the peptide backbone and the copper ion it coordinates with the copper contributing to enzymatic cofactor activity and oxidative stress modulation

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The 2017 Neelakantan paper in Biochemistry characterised a fluorescent assay for direct real-time monitoring of NNMT enzymatic activity, providing the analytical foundation for inhibitor screening including 5-Amino-1MQ (PMID 28121423)